AlgaTech^®The high-throughput algae phenotype imaging analysis platform adopts PTS (Plant To Sensor) automatic transmission technology, integrating international advanced spectral imaging technologies such as hyperspectral imaging and chlorophyll fluorescence imaging. The samples are automatically transmitted to the corresponding imaging workstations through the transmission platform, achieving high-throughput, non-destructive hyperspectral imaging, chlorophyll fluorescence imaging, and multispectral fluorescence imaging analysis. It is currently a fully functional and technologically advanced algae phenotype imaging analysis system in China, which can provide comprehensive, one-stop, high-throughput solutions for algae phenotype analysis, physiological and ecological research, germplasm resource detection research, genetic breeding, resistance screening, photobiology research and other fields.

AlgaTech, Ocean University of China^®Platform installation, debugging, and experimental operation

Main technical features:

ØOne stop algae phenotype imaging analysis platform, integrating VISIR-NIR hyperspectral imaging, chlorophyll fluorescence imaging, multispectral fluorescence imaging technology, and optional Thermo RGB imaging analysis

ØPTS(Plant to Sensor) technology platform, dual track synchronous lifting control SpectraScan^©High precision mobile scanning platform, samples can be placed on the precise displacement platform and automatically transported to the imaging unit for imaging analysis

ØUV, cold white, red, blue, green, cyan and other different excitation light sources can be selected to adapt to multispectral fluorescence imaging analysis of different algae with different excitation light

ØIt can perform imaging detection and analysis of phenotypic traits of macroalgae such as kelp, microalgae (placed in culture dishes or porous plates, etc.), algae attached to media such as corals, and soil algae

ØModular structure design with powerful system expansion capabilities, capable of remote control and automatic operation of data collection and storage

ØTouch screen control, embedded operating system, fully Chinese ground station software, wireless control platform operation

ØBuilt in temperature and humidity, light intensity, clock (clock can synchronize computer time with one click or automatically calibrate based on GPS information)

ØSupport combination commands (Protocols) to automatically run protocols

ØThe host system comes with casters, making it easy to move and suitable for working environments such as laboratories and greenhouses

Main technical indicators:

1)Hyperspectral imaging station: standard configuration includes 400-1000nm visible light near-infrared and 900-1700nm short wave infrared hyperspectral imaging analysis, optional 1000-2500nm SWIR hyperspectral imaging sensor

a)Number of bands: 224 channels

b)Spectral resolution: FWHM 5.5nm（400-1000nm）、8nm（900-1700nm）

c)Spatial resolution: 1024x (400-1000nm), 640x (900-1700nm), optional with other resolutions for hyperspectral imaging

d)Signal to noise ratio 600:1 (400-1000nm), 1000:1 (900-1700nm)

e) Measurement parameters: Imaging measurement and analysis of physiological and biochemical indicators, light use efficiency, health index, chlorophyll and carotenoid content, stress and other parameters.

Engineers from Yiketai Spectral Imaging and Unmanned Aerial Vehicle Remote Sensing Research Center conducted hyperspectral imaging analysis and chlorophyll fluorescence imaging analysis on cultivated seaweed and algae in culture dishes on site (the right figure shows the multispectral fluorescence imaging analysis of seaweed, quoted from: Lei Tang, Liping Qiu, Cong Liu, Guoying Du,Zhaolan Mo, Xianghai Tang,Yunxiang Mao.Transcriptonic Insights into Innate Imunity Responding to Red Rot Disease in Red AlgaPyropia yezoensis. Molecular Sciences,2019）

2) Chlorophyll fluorescence imaging station:

a) Professional high-sensitivity chlorophyll fluorescence imaging CCD, frame rate 50fps, resolution 720x × 560 pixels, pixel size 8.6 × 8.3 µ m

b) Photochemical light up to 1000 µ mol.m-2 S-1 adjustable, saturated pulse 3900 µ mol.m-2 s-1

c) Can automatically run protocols such as Fv/Fm, Kautsky induction effect, fluorescence quenching analysis, light response curve, etc

d) More than 50 chlorophyll fluorescence automatic measurement and analysis parameters, including Fv/Fm, Fv '/Fm', Y (II), NPQ, qN, qP, Rfd, ETR, etc., automatically generate chlorophyll fluorescence parameter maps

e) Automatically synchronize the display of chlorophyll fluorescence parameters and parameter graphs, chlorophyll fluorescence dynamic curves, and chlorophyll fluorescence parameter frequency histograms

3)Multispectral Fluorescence Imaging Station: UV excited multispectral fluorescence imaging,Reflect the dynamic changes of secondary metabolites such as polyphenols and flavonoids, chlorophyll, plant aging, plant pest and disease stress, and abiotic stress

a)High resolution CCD lens, 1392x1040 pixels, effective pixel size is6.45μm，Pixel binning can be used to improve sensitivity (2x2, 3x3, 4x4)

b)7Position filter wheel and filter, used for imaging measurement of multispectral fluorescence F440, F520, F690, F740 and other biological fluorescence phenomena

4)Automatic measurement and analysis function (unmanned): One or two test programs can be preset, and the system can automatically measure and store them. For example, during the day, the Kautsky induction effect program can be automatically timed to run, and at night, the fluorescence quenching analysis program can be automatically timed to run

5)Optional GFP/YFP steady-state fluorescence imaging or LUC luciferase imaging

6)Optional UV, red, green, cyan, blue, far red and other light sources in different frequency bands

7)Chlorophyll fluorescence imaging and multispectral fluorescence imaging tools have menus such as Live (live testing), Protocol (experimental program selection), Pre processing (imaging preprocessing), Result (imaging analysis results), etc. The Protocol experimental program can be freely edited, and customers can also use the wizard program template in the Protocol menu to freely create new experimental programs

8)Infrared thermal imaging (optional):

a)Resolution: 640 × 512 pixels, optional with other high-resolution infrared thermal imaging sensors

b)Measurement temperature range:-25℃－150℃

c)Sensitivity: 0.03 ℃ (30mK) @ 30 ℃

d)Spectral range:7.5－13.5μm

e)Sensor: Uncooled infrared focal plane sensor, calibrated at multiple points (with calibration certificate)

f)1-142x digital zoom

g)The software has a color palette (14 color combinations including natural, rainbow, grayscale, gradient, etc.), difference technology, temperature range setting (to change color distribution or highlight selection range, etc.), isotherm mode, ROI selection analysis, temperature scanning (displaying temperature distribution curve of selected lines, etc.), profile temperature, time chart, etc; Can display image information; Having reporting modes, etc;

9)RGBImaging: High sensitivity, 1-40 times magnification, capable of micro and macro imaging analysis, optional higher resolution imaging unit

Yiketai Ecological Technology Company provides comprehensive technical solutions for phenotype analysis of algae and marine plants:

1)Algae cultivation and online monitoring technology

2)SpecimHyperspectral imaging analysis technology

3)FluorCamChlorophyll fluorescence imaging analysis technology

4)FluorCamMultispectral Fluorescence Imaging Analysis Technology

5)FKMMicroscopic Chlorophyll Fluorescence Imaging and Spectral Analysis

6)AlgaTech^®One stop high-throughput algal phenotype imaging analysis platform

7)Scheme for Chlorophyll Fluorescence Thermoluminescence Measurement Technology of Algae

8)Algae photosynthesis, chlorophyll fluorescence, hyperspectral measurement portable case

9)Ecodrone^®Unmanned aerial vehicle remote sensing technology for remote sensing analysis of marine aquaculture

10)Ecolab^®Laboratory technology cooperation and technical service plan